AggreGuard improves productivity, an eGFP model

AggreGuard™ facilitates reduced upstream process optimisation and increases eGFP production post-transduction

Cell-to-cell adhesion is the main driver of cell clumping and microcarrier aggregation in suspension-based agitated bioreactors.  CellRev tested a hypothesis: reducing cell-to-cell adhesion with AggreGuard™ in a representative upstream bioprocess can improve the viral titre while maintaining cell culture homogeneity without cell line engineering or media formulation. This hypothesis was evaluated in 125 mL disposable Corning® spinner flasks using an enhanced green fluorescent (eGFP) protein expressing adeno-associated viral vector to transduce Vero cells, mimicking a viral infection.

Results showed that a reduction in cell-to-cell adhesion with AggreGuard™

1. increased eGFP expression ~3-fold;
2. resulted in a visually a more homogenous cell culture; and

3. yielded a 1.5-to-2-fold more viable cells harvested at process end.

    

Read our full case study here > https://cellrev.co.uk/case-study-aggreguard-improves-productivity-an-eg…